Authors
Regina Widiandani¹ | Wendry Setiyadi Putranto² | Fatkhanuddin Aziz³ | Sarasati Windria^1,4

Affiliations

¹Study Program of Veterinary Medicine, Padjadjaran University, Sumedang, Indonesia | ²Department of Animal Products Technology, Faculty of Animal Husbandry, Padjadjaran University, Sumedang, Indonesia | ³Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia | ⁴Department of Biomedical Sciences, Division of Microbiology, Veterinary Medicine Study Program, Faculty of Medicine, Padjadjaran University, Sumedang, Indonesia

Background

• Staphylococcal enterotoxins from S. aureus cause food poisoning in humans.

• Nonclassical enterotoxins are gaining attention for their potential role in food safety.

Methods

• 22 stored S. aureus isolates from milk of cows with subclinical mastitis at a farm in Sumedang, Indonesia.

• Eight nonclassical enterotoxin genes were screened by multiplex PCR.

Key Results

• The sek gene was detected in 90.9% (20/22) of isolates.

• The sep gene was found in 4.5% (1/22).

• No amplification was observed for the remaining genes.

Discussion & Conclusion

• Enterotoxin-associated genes in mastitis milk isolates may matter at the animal–food interface.

• The findings provide baseline data for molecular surveillance; PCR shows gene presence, not expression or virulence.

Related SDGs

SDG 2: Zero Hunger – Supporting safer dairy production

SDG 3: Good Health and Well-Being – Protecting consumers from foodborne illness

Journal Information

Journal: Scientific World Journal

DOI: https://doi.org/10.1155/tswj/3410756